A rodent model of impaired gastric motility resulting from gastric inflammation induced by allyl isothiocyanate, a pungent ingredient of wasabi, to evaluate prokinetic agents

Abstract

AIM: We attempt to establish a rodent model of impaired gastric motility resulting from gastric inflammation by allyl isothiocyanate (AITC), which is reliable produce to evaluate prokinetic agents. METHODS: Gastric inflammation: The rat stomachs were mounted on ex-vivo chambers under anesthesia. Vascular permeability was measured in response to AITC. Motility: Gastric motility was measured by ¹³C-acetic acid breath test in conscious mice after AITC. RESULTS: AITC increased vascular permeability in rat stomachs in concentration-dependent manner. Yet, AITC-induced vascular permeability was not inhibited by a TRPA1 blocker A-967079. AITC impaired gastric motility in conscious mice. The decreased gastric motility in response to AITC was restored by prokinetic agents including itopride, mosapride, neostigmine, and a functional dyspepsia (FD) therapeutic agent acotiamide. Gastric damage was not observed. CONCLUSIONS: it is suggested that AITC induced gastric inflammation in the rodent stomach resulting in impaired gastric motility that is reversible by prokinetic agents. This rodent model could be useful for developing the new prokinetic agent in FD.