Abstract
【Subject】It is suggested that aging and excessive intake of fat may induce dysbiosis and intestinal inflammatory damage additively. Recent studies reported that molecular hydrogen has anti-inflammatory effect and antioxidant effect. Therefore, we analyzed the effects of molecular hydrogen on dysbiosis and intestinal injury induced by aging and excessive intake of fat.
【Method】Senescence-accelerated mouse prone-8 (SAMP8) were fed control diet or high fat diet (HFD) for 14 weeks, and then the each group was fed placebo jelly or hydrogen-rich jelly for 4 weeks. After the treatment, small intestinal tissues were harvested for morphological examination as well as organoid analysis. Moreover, we analyzed alterations of microbiota composition in cecal feces by 16S rRNA gene analysis of microbiota profiling.
【Result & Conclusion】The treatment with hydrogen-rich jelly prevented intestinal morphological damage and suppressed the decreases of CDX2 and BrdU expression in HFD-loaded SAMP8 mice. The treatment also increased the number of cultured organoids derived from small intestine of HFD-loaded SAMP8 mice. The 16S rRNA gene sequencing analysis suggested that the treatment decreased the abundance of Proteobacteria phylum in HFD-loaded SAMP8 mice. These findings suggest that the treatment with molecular hydrogen may modify microbiota composition and suppress intestinal injury and regeneration dysfunction.
