Host: The Japanese Pharmacological Society
Name : The 95th Annual Meeting of the Japanese Pharmacological Society
Number : 95
Location : Fukuoka
Date : March 07, 2022 - March 09, 2022
[Introduction]
Dog bladder cancer is almost a muscle-invasive type of cancer, and many have poor prognoses due to low sensitivity to chemotherapy and difficulty in complete surgical resection. However, the obvious cause and mechanism, as well as effective treatment and prevention, have not yet been identified. Three-dimensional organoid culture is a method that can reproduce three-dimensional epithelial tissue structures of various organs on culture dishes. So far, human and mouse bladder organoids have existed, but organoids from healthy dogs have not yet been established. Therefore, we conducted this study with the aim of applying them to various researches, such as understanding the development mechanism of bladder cancer in dogs.
[Methods and Results]
The established organoids showed stable growth without bacterial contamination and were observed as spherical morphology. The organoids reproduced the layered structure of the uroepithelium, expressed uroepithelial markers such as CK7 and UPKIIIA, and showed different drug sensitivity among strains. In addition, histopathological images, dependence on culture medium components, gene expression, and protein expression were different from those of canine bladder cancer organoids.
[Conclusion]
In this study, canine normal bladder organoids were produced using a minimally invasive sample collection method, and continuous culture was possible. The organoids have a structure similar to that of the bladder in vivo, suggesting that they are useful as an experimental model of the canine normal bladder.
