Host: The Japanese Pharmacological Society
Name : The 96th Annual Meeting of the Japanese Pharmacological Society
Number : 96
Location : [in Japanese]
Date : November 30, 2022 - December 03, 2022
Diabetes mellitus and brain toxicity are closely linked. Oxidative stress, obesity, insulin resistance, and glucose toxicity can affect the brain. Orexin-A, also known as hypocretin-1, participates in many physiological processes through its activated receptor. Orexin-A has been associated with feeding behavior, obesity, and pathogenesis of Alzheimer's disease. We reported that high-dose thiamine in obese diabetic Otsuka Long–Evans Tokushima Fatty (OLETF) rats leads to reduced obesity and metabolic disorders. In addition, we found that plasma orexin-A levels in OLETF rats can be modulated by thiamine supplementation under conditions of oxidative stress. Herein, we focused on orexin-A in obese diabetic OLETF rats. At 58 weeks of age, the rats showed an increase in body weight and blood glucose levels. Plasma orexin-A was measured by ELISA and tended to be higher in obese diabetic OLETF rats than in non-obese diabetic control rats. We evaluated hypocretin receptor 1 (Hcrtr1, also orexin-A receptor) gene expression in the brain of diabetic OLETF rats by reverse transcription-polymerase chain reaction and found that diabetic OLETF rats exhibited higher orexin-A receptor gene expression in the brain than controls. The results presented here are expected to provide a better understanding of the role of orexin-A and its contribution to diabetic brain.
