Host: The Japanese Pharmacological Society
Name : The 96th Annual Meeting of the Japanese Pharmacological Society
Number : 96
Location : [in Japanese]
Date : November 30, 2022 - December 03, 2022
Axons in the central nervous system (CNS) are wrapped with myelin formed by oligodendrocytes. CNS damage causes demyelination which leads to neurological dysfunction. Remyelination is a regenerative process, which is mediated by oligodendrocyte precursor cells (OPCs) development. Mechanisms underlying OPCs development have been extremely investigated with in vitro models using 2-dimension (2D) culture systems. Although 3D culture easily offers model mimicking physical and mechanical properties of cell adhesion or microenvironment in vivo, little is known about the difference of OPCs properties between in 2D and in 3D culture. Here, we characterized the phenotypic difference of OPCs cultured in between 2D and a collagen-based 3D culture system. OPCs isolated from murine mixed glial culture were embedded in type I collagen gel (3D, final concentration: 2.4 mg/mL) or plated on glass substrate (2D), respectively. Immunocytochemical analysis showed a significantly low level in the percentage of Ki67+ cells in Olig2+ cells in 3D compared with 2D, suggesting that OPCs have less proliferative capacity in 3D. When differentiation was induced by triiodothyronine, a low level of the expression of myelin basic protein was observed in 3D compared to 2D, suggesting that OPCs have less differentiation capacity in 3D. Collagen gel can easily control the stiffness by change in concentration, therefore, these results provide crucial insights to understand OPCs response in 3D culture, which contributes to develop a 3D model mimicking pathological conditions.
