Comparison of fibrosis levels and drug effects on fibrosis among various mouse models of ischemic acute kidney injury

Abstract

【Background and Purpose】The number of patients who progress from acute kidney injury to chronic kidney disease is increasing. Glomerulosclerosis and fibrosis of the renal tubular interstitium are commonly observed in end-stage renal disease. In this study, we compared the fibrosis levels after ischemia-reperfusion treatment in mice with acute kidney injury to develop a pathological model suitable for drug evaluation. The effects of drugs on fibrosis after ischemia-reperfusion were also examined.【Methods】Male C57BL/6J mice were divided into three groups: the left kidney ischemia for 25 min group (left kidney ischemia), left kidney ischemia for 25 min and right kidney removal one week later group (left kidney ischemia-right nephrectomy), and left and right kidney ischemia for 25 min group (left and right kidney ischemia). The fibrosis levels after ischemia-reperfusion treatment were compared.【Results】All groups showed an increased kidney hydroxyploline and area fraction of positive Sirius Red stain. The degree of increase in hydroxyploline and Sirius Red stain positivity was similar in the left ischemia-right nephrectomy group and the left and right ischemia group, and was more than twice as high in the left ischemia group compared to the two groups. Mixed feeding of pirfenidone significantly suppressed fibrosis after ischemia-reperfusion treatment.