Host: The Japanese Pharmacological Society
Name : The 97th Annual Meeting of the Japanese Pharmacological Society
Number : 97
Location : [in Japanese]
Date : December 14, 2023 - December 16, 2023
【Background】Cholesterol-induced cholelithiasis is the most popular diseases in all of cholelithiasis. Cholesterol gall stones are generally formed caused by various factor following unbalance cholesterol secretion. Recently, it has been reported that there are some functional changes of gall bladder like mucus secretion or motility as one of the factors for cholelithiasis, but there has not been established the mechanism related to the functional changes of gall bladder mucosa. Additionally, organoid is often used for the pathological evaluation because it can reproduce cell composition, characteristics, and function, but it has not been established organoid model for cholelithiasis.
【Objectives】We established gall bladder organoid derived from cholesterol-induced cholelithiasis model mouse and evaluated the functional disorder mechanism for cholesterol-induced cholelithiasis.
【Methods】We feeded inducing diet to 4 weeks or 8 weeks mice, and produced cholesterol-induces cholelithiasis model mice. Using extracted tissue, we evaluated the pathological characteristics about gall stones formation and gall bladder structure. And also, we cultured gall bladder organoid derived from extracted tissue and compared its structure or mucus production with original tissue. Additionally, the gene expression difference was analyzed by RNA sequence.
【Results】It was observed the gall stones formation in 4 weeks feeding mouse. Organoid size in 4 weeks feeding mouse was significantly bigger than control group. In 8 weeks feeding mouse, organoid size was also increased significantly and gallbladder inflammation or gallbladder wall hypertrophy were recognized in gallbladder tissue. Furthermore, in RNA sequence, there were genetic expression differences between control and feeding group.
【Conclusion】It was suggested that gall bladder organoid derived from cholesterol-induced cholelithiasis model mouse can reproduce pathological characteristics of cholelithiasis. We will plan to do more functional analysis based on RNA sequence.
