Host: The Japanese Pharmacological Society, The Japanese Society of Clinical Pharmacology
Name : WCP2018 (18th World Congress of Basic and Clinical Pharmacology)
Location : Kyoto
Date : July 01, 2018 - July 06, 2018
Bulbine abyssinica A. Rich has been reported to possess a variety of pharmacological activities traditionally. Previous work suggested its antidiabetic properties but there is paucity of data in literature on the bioactive compounds.
The present research exertion was aimed to isolate and identify biologically active polyphenols from B. abyssinica and evaluate the efficacy on the carbohydrate digesting enzymes.
Polyphenolics from the methanolic extract of B. abyssinica leaves were isolated by the silica gel column chromatography to yield different fractions. The antioxidant activiries of these fractions were carried out against 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2'-diphenyl-2-picrylhydrazyl (DPPH) radicals and ferric reducing antioxidant power (FRAP).
In-vitro antidiabetic experimentation was performed by evaluating the α-amylase and α-glucosidase inhibitory capacity. The isolated polyphenols were then identified using liquid chromatography and Mass spectroscopy (LC/MS).
The results showed eight polyphenolic fractions (BAL 1-8), BAL-4 has the highest inhibitory activity against ABTS radicals whereas BAL-6 showed potent ferric ion reducing capacity. BAL-5 was the most effective fraction with antidiabetic activity with IC50 of 140.0 and 68.58 ± 3.2 ug/ml for α-amylase and α-glucosidase inhibitory activities respectively. The LC/MS analysis revealed the presence of carvone in all the fractions. It was concluded that the present study demonstrates the antioxidant and anti-diabetic activities of the isolated polyphenols from B. abyssinica.
