TELMISARTAN TREATMENT IMPROVES BONE MARKERS EXPRESSION ON MANDIBLE OF SPONTANEOUSLY HYPERTENSIVE RATS WITH PERIODONTAL DISEASE

Abstract

Background

Periodontal disease (PD) is an inherited or acquired disorder of tooth surrounding tissues and alveolar bone initiated by bacteria biofilm accumulation. Coexistence of systemic conditions such as hypertension can lead to exacerbated inflammatory response and enhanced bone resorption. Recent studies have demonstrated that local renin angiotensin system may have a role on PD progression.

Aims To evaluate the effects of telmisartan Telm Angiotensin II receptor type 1 blocker on gene expression of bone metabolism markers on mandibles of normotensive and hypertensive rats with experimentally induced PD.

Methods 10 week old male Wistar and SHR were subjected to 15 days of PD induced by bilateral silk ligature placed on the first inferior molars and were concomitantly treated with Telm 10 mg Kg. Experimental groups were labeled as Control C PD and PD+Telm of Wistar W and SHR S animals. Hemimandibles were harvested for real time RT PCR analysis of bone formation markers Runx2 Osterix Beta catenin osteoprotegerin BMP 2 alkaline phosphatase osteocalcin osteopontin and bone sialoprotein and bone resorption remodeling markers tartrate resistant acid phosphatase RANK RANKL cathepsin K MMP 2 and 9 and OSCAR. The protocol was approved by Institutional Animal Care and Use Committees School of Dentistry of Aracatuba Process 006862016.

Results PD did not significantly alter the expression of transcription factors but Telm was able to increase Runx2 expression. Regarding bone formation PD markers significantly reduced Alp expression and Telm treatment increased its expression especially in SHR. Opg Rankl Rank axis had increased expression in groups with PD and Telm treatment reduced Rankl expression only in SHR. Bone resorption markers as Trap Mmp9 Ctsk Oscar Vtn and Itga5 were elevated by PD on both strains and Telm treatment was able to prevent this response.

Conclusion Our results suggest that Telm treatment may have a beneficial effect on PD progression by increasing expression of bone formation markers preventing the increased expression of bone resorption markers and possibly reducing alveolar bone loss probably by blocking local angiotensin II effect on periodontal tissues. Acknowledgment FAPESP Grant 2015039652 and CAPES for financial support.