FOOD IRRADIATION, JAPAN
Online ISSN : 1884-3611
Print ISSN : 0387-1975
ISSN-L : 0387-1975
Quantitative Determination of Chlorogenic Acid in γ-Ray-Irradiated Potato Tubers by High PerformanceLiquid Chromatography
Makoto NISHIZAWATakao CHONANTakashi YAMAGISHIYoshiaki ANDO
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1984 Volume 19 Issue 1-2 Pages 1-8

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Abstract
chlorogenic acid (CGA) and caffeic acid (CA) in lyophilized potato tubers were quantitatively determined by high performance liquid chromatography (HPLC), and the changes in CGA contents of γ-ray-irradiated potato tubers (100 Gy) during storage at 25°Cwere studied.
The CGA contents of the irradiated potato tubers slightly increased immediately after irradiation, but decreased gradually after 29th day and reached the same level as that in the beginning of storage on the 73rd day after irradiation. However, the CGA contents of the unirradiated potato tubers increased steadily with increasing days of storage period, and reached twice that in the beginning of storage on the 73rd day after irradiation.
The CGA contents in potato tubers determined by Diazo and Folin-Denis methods were shown to be two - eight times higher than those by HPLC method, and there appeared to be no significant relation between the CGA contents determined by HPLCmethod and by colorimetrical methods.
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© JAPANESE RESEACH ASSOCIATION FOR FOOD IRRADIATION
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