DNA content in bull spermatozoa during storage in yolk-citrate diluent at 4°C
1965 Volume 10 Issue 4 Pages 109-113
Details
1965 Volume 10 Issue 4 Pages 109-113
1. Microspectrophotometric observations have been made of the DNA content of bull spermatozoa during storage in yolk-citrate diluent at 4°C to reexamine the earlier results by SALISBURY et al1). 2. It was found that hydrolysis for twelve minutes was improper to sperm DNA. The optimum hydrolysis time with IN-HCI at 60°C was about 7 minutes. 3. There was a significant difference in the head area of spermatozoa between live and dead cells, judged by Eosin staining technique. In dead spermatozoa, the increase of the head area was recognized. 4. The amount of Feulgen-DNA in bull spermatozoa decreased significantly after 5th day of the storage when examined at 0, 5th, 10th and 19th days.
