Abstract
The anti-PMSG serum were reacted to PMSG preparation and gonadotropin in pregnant mare serum. The reaction with anti-PMSG serum to HCG, FSH, HGH, ACTH and various animal sera were observed to be all negative. Immunological activity in sera of 264 pregnant mare was titer 4. The false negative cases of appearance of immunological pregnancy test were directly related to the concentration of the gonadotropin in pregnant mare serum.
The in vivo neutralization of PMSG by anti-PMSG serum was demonstrated. The materials to test the ability of antibody to inactivate PMSG were injected into 21-28 days immature female mice. No corpora lutea were observed in the fraction from ether solution, 100 IU/ml of PMSG in saline with anti-PMSG serum, precipitate containing the hormone-antibody complex and pregnant mare serum with anti-PMSG serum. While 100 IU/ml of PMSG in saline with normal rabbit serum, 100 IU/ml of PMSG in saline and pregnaut mare serum were showed prominent luteinization.
