Measurement of PMSG in serum of horse with the heamaggrutination inhibition reaction

Abstract

It was investigated on the posibility of the measurement by means of the heamagglutination inhibition reaction (HAIR) of the PMSG in horse serum and the application of early diagnosis for pregnancy with HAIR. The results were summarized as follows.
1) The PMSG protein and non-specific substances in horse serum are fractionated and separeted by neutralization with meta phospholic acid-alkali. The activity of the PMSG was roughly recovered quantitatively with this method.
2) The antigen-antibody reactions was observed among each antigen to the PMSG and between the PMSG and anti-PMSG, anti-ES by the cross-HAR. This seems to be indicated that the protein from host origin coexist as impure protein into protein substance of hormone.
The anti-ES factor was eliminated from antigen-antibody systems between the PMSG and the anti-PMSG by reaction with male horse serum lyophilized. In cross HAIR and the neutralization tests on the hormone activity, it showed specifically the positive reactions between the PMSG and anti-PMSG, while the reactions with the HCG, ES, HS and NRS were not observed.
3) It is found that the comparisons between the HAIR and the biological response shows the agreed results.
4) It was investigated on the development and persistence of the PMSG in serum with the lapse in pregnancy of horse. The time that the PMSG is demonstration in serum differs from individuals, and it can not obtained the PMSG in serum within 30 days after the fertilization. But after that, the PMSG value increased gradually and indicated the largest titer at 6090 days, but it showed the decrease with progress pregnant periods, and the PMSG titer in each horses was not obtained since 140 days after fertilization. The differences among individuals, however, was large considerably.
5) On the posibility of early diagnosis for pregnancy using the PMSG in horse serum by means of the HAIR, the applied investigation in field are performed in order to compare with biological response.
From these results, all horse serum that the biological response was negative were also negative in the HAIR. And the positive in both biological response and the HAIR indicated the high and agreed rate in 92.4%, but 7.6% of which showed susceptible positive or negative.
It seemed to be necessary to examine individual horses on these causes.