Abstract
An enzymohistochemical demonstration was carried out using rabbit ovaries obtained 10 days after mating from normally pregnant, and pregnant or non-pregnant rabbits after superovulation treatment. The results are as follows.
Corpora lutea from normally pregnant rabbits showed week or very slight hydroxysteroid dehydrogenase (OH-SDH) activities: activities of 3α-OH-SDH (NAD dependent), Δ5-3β-OH-SDH (DNA as a subtrate, NAD, NADP ; pregnenolone, NAD), 11β-OH-SDH (NAD), 16β-OH-SDH (NADP) and 17β-OH-SDH (NAD) were weak, while those of 3α-OH-SDH (NADP), Δ5-3β-OH-SDH (pregnenolone, NADP), 11β-OH-SDH (NADP), 16β-OH-SDH (NADP) and 17β-OH-SDH (NADP) were weaker, or slight. As for other dehydrogenases (DH), activities of G-6-PDH and MDH (NAD) were intense, those of SuDH and LDH were moderate, of MDH (NADP) and α-GDH were weak, and of β-GDH and GDH were slight. Activity of acid phosphatase was moderate, while that of non-specific esterase (Etase) was weak.
Corpora lutea from superovulated pregnant rabbits exhibited almost the same enzyme activities as those from normally pregnant rabbits, except for the decrease shown in LDH and Etase activities. On the other hand, corpora lutea from superovulated but non-pregnant rabbits showed weaker activities, in the OH-SDH that showed weak but plus activities concerning pregnant rabbits. They showed weaker activities in SuDH, MDH (NAD) and α-GDH also. Blood follicles that appeared in treated rabbits showed similar enzyme activities to corpora lutea.
Interstitial tissue, too, were observed concerning about the same enzymes as above.
