Abstract
Sugar constituents in bovine uterine fluid were studied by gas-liquid and thin-layer chromato-graphies.
The uterine fluid was collected at slaughter from thirty healthy cows during estrous cycle and from four cows at 3040 days of pregnancy. Preparation for the chromatographies was made by deproteinizing and freeze-drying the fluid. Either trimethylsilylation or acetylation was further necessary for the gas-liquid chromatography.
Using columns SE-52, QF-1 and OV-17 in the gas-liquid chromatography and Kieselgel G in the thin-layer chromatography, presence of glucose, fructose, sorbitol and inositol was confirmed in the fluid during estrous cycle and preplacentaion stage of pregnancy. Other carbohydrates such as rhamnose, xylose, galactose, erythritol, xylitol, adonitol and ascorbic acid were not detectable.
