Studies on the uterine secretion of the cow
VII.Sugar and sugar alcohol concentrations in luminal fluid and tissue of bovinegenitalia during estrous cycle
1973 Volume 19 Issue 1 Pages 10-15
Details
1973 Volume 19 Issue 1 Pages 10-15
Fructose, glucose, sorbitol and inositol concentrations were determined on the luminal fluid and tissue in bovine genitalia at various stages of estrous cycle.
The genitalia was obtained from 23 healthy dairy cows and heifers, and 10 beef heifers at slaughter.
Samples collected for analysis were uterine fluid, cervicalmucus, washings of oviduct, and tissues of isthmus of uterine tube, carunclar area, intercarunclar endometrium and cervix.
They were deproteinized by Somogyi's method, lyophilized, trimethylsilylated and subjected to gas-liquid chromatography for carbohydrate assay. The chromatographic separation was carried out on a 5% SE-52 on Shimalite W column (3 m x 3 mm i. d.) by linear temperature-programmed analysis. Column oven was conditioned for a temperature increase of 1°C per minute from 165°C. Detector oven was maintained at 330°C and injection port at 300°C. A nitrogen carrier gas was used at flow rate 60ml/min. The hydrogen flow to the flame jet was 45 ml/min.
The concentration of the materials was estimated using adonitol as an internal standard.
The results obtained were asfollows.
1. The levels of fructose, glucose, sorbitol and inositol in uterine fluid showed a definite variation with the stage of estrous cycle.
The each concentration of four constituents was low at estrus and after ovulatoan, and increased at early luteal stage. The high levels were maintained until onset of the nextestrus. The ratios of the constituent levels at mid-luteal stage to those at estrus were 15 in fructose, 6 in glucose, 346 in sorbitol, 18 in inositol.
The main carbohydrate was glucose at estrus and sorbitol during luteal phase.
2. Fructose, glucose, sorbitol and inositol were also found in the cervical mucus. Their levels were lower than those in uterine fluid at every stage in estrous cycle.
In the tissue of genitalia, sorbitol occurred only in endometrium during luteal stage. Therefore, sorbitol in the cervical mucus might be due to uterine fluid discharged from the uterine cavity.
3. In the oviduct, active secretion of inositol was found out, but secretion of sugars was uncertain.
