Abstract
Effects of various doses of PMS and HCG on ovulation, implantation and foetal mortality in the mouse were studied. Results obtained were as follows.
1) The ovulation rate was not significantly different between various combinations of PMS and HCG in the experiment where copulating stimuli were given simultaneously (Table 1).
Higher the dose of PMS, however, larger number of ova was ovulated. The maximal ovulation was induced with 10 IU of PMS followed by 10 IU of HCG.
2) Implantation sites were inspected in the mice killed on 18 day gestation. Pregnancy rate (judged by presence of implantation site) was lower than that of control mice, and treatment with 10 IU of PMS and any doses (110 IU) of HCG was less effective than others.
Maximal implantation (21.7 & 21.6 sites) was observed by 5 IU of PMS (Table 2).
3) Larger number of living foetuses (12.617.3) was obtained by 5 IU of PMS compared with 10.9 in control gruop.
Foetal mortality tend to increase by higher dose of PMS and HCG (Table 2).
4) In conclusion, it was suggested that the most effective dose for implantation and fortal living in superovulated mice was 5 IU of PMS and 110 IU of HCG.
