Abstract
Three females born with acardius amorphus in cattle were cytogenetically examined. Two females showed the sex chromosomal chimera (XX/XY), but the other female did not show any aberrations. The present study indicated that freemartinism can also occur in females born with acardius amorphus, in addition to the females of heterosexual twins and multiple births or single-born animals. The acardius amorphus and its twin were found to be dizygotic twins of different sex with XX/XY chimerisms.