Host: Society for Reproduction and Development
Name : The 112th Meeting of the Society for Reproduction and Development
Number : 112
Location : [in Japanese]
Date : September 02, 2019 - September 05, 2019
Anti-Müllerian hormone (AMH) is a glycoprotein, and its blood concentration can indicate fertility in various animals, but little is known for physiological mechanisms. Its most well-known secreting organ is ovaries, where AMH has the important roles. The main receptor of AMH is expressed in bovine oviducts and endometria (our unpublished data). Ovaries, oviduct, and endometria exchange various hormones using the systemic circulation or counter-current transfer. Therefore, this study investigated whether AMH is expressed in bovine oviducts and endometria. Reverse transcription-polymerase chain reaction and western blotting detected expressions of AMH mRNA and protein in oviductal and endometrial samples. Immunohistochemistry revealed robust AMH expression in the tunica mucosa of the ampulla and isthmus, and in the grandular and luminal epithelium of endometrium. We compared AMH mRNA (measured by real-time PCR) and protein expression in the ampulla, isthmus, and endometrium among old Holsteins, postpubertal heifer Wagyus, and old Wagyus. The heifer Wagyus had lower AMH expression in ampulla than old Holsteins (P < 0.05), and lower AMH expression in the isthmus than both old Holsteins and Wagyus (P < 0.05), but not in endometrium (P > 0.1). In conclusion, AMH is expressed in bovine oviducts and endometria.
