Development of DNA Detection Method after Agarose Gel Electrophoresis Suitable for Environmental Education

Abstract

DNA detection methods by staining agarose gels after electrophoresis were evaluated to find safer ones suitable for experiment on environmental education. Detection method using dye and visible rays is thought to be safer than the method using ethidium bromide and ultraviolet rays. Three methods using dye and visible rays: staining by（a）ViewaBlue^Ⓡ Stain,（ b）stains-all, or（ c）indoine blue and methyl orange, were compared with detection method using ethidium bromide. These detection methods were less sensitive than that using ethidium bromide. In the methods using dye and visible rays, stains-all method was the most sensitive.Moreover, costs of each methods were estimated for educational use.