Expression Changes of ERK1/2, STAT3 and SHP-2 in Bone Marrow Cells from γ-ray Induced Leukemia Mice

Abstract

The aim is to clarify expression changes of ERK1/2, STAT3 and SHP-2 in bone marrow cells from γ-ray induced leukemia mice. A mouse model of γ-ray induced leukemia was produced, and by means of quantitative real-time PCR, immunoprecipitation, Western blotting and electrophoretic mobility shift assays (EMSA), the expression of mRNA and protein, phosphorylation level, and protein activity of ERK1/2, STAT3 and SHP-2 in bone marrow cells were investigated in these mice. The results indicated that mRNA and protein expressions of ERK1/2 were upregulated, with significant increase of phosphorylation level and protein activity, but with insignificant differences in mRNA and protein expressions, phosphorylation level and protein activity of STAT3 and SHP-2 in bone marrow cells from γ-ray induced leukemia mice compared to the radiation/tumor-free or control mice. It is concluded that in the pathogenesis of γ-ray induced leukemia in Balb/C mice, activated ERK1/2 pathway may play a role, without involving STAT3 pathway; meanwhile, SHP-2 exerts no regulative effect on pathways of Ras-ERK1/2 and JAK-STAT.