In Vitro Radiosensitivity of Human Fresh T-lymphocytes by Colony Formation Assay Using PHA and Recombinant Interleukin-2

KAORI TATSUGAWA; NORI NAKAMURA; MASAYUKI HAKODA; MITOSHI AKIYAMA

doi:10.1269/jrr.28.221

KAORI TATSUGAWA, NORI NAKAMURA, MASAYUKI HAKODA, MITOSHI AKIYAMA

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Keywords: Radiosensitivity, Ionizing radiation, Cell killing, Human T-lymphocytes

JOURNAL FREE ACCESS

1987 Volume 28 Issue 3 Pages 221-226

DOI https://doi.org/10.1269/jrr.28.221

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Abstract

In vitro culture conditions for colony formation of human fresh peripheral T-cells using PHA and recombinant Interleukin-2 are defined. Peripheral lymphocytes, from six individuals, were exposed to X or gamma rays in vitro, and dose-survival curves were obtained. The results showed typical sigmoid curves similar to those observed when other mammalian cells are exposed to radiation. The D₁₀ (dose required to kill 90% of the cells) was found to be 3.0 to 3.5 Gy.

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