Effects of X-ray Irradiation on Genomic DNA Methylation Levels in Mouse Tissues

RIICHI TAWA; YUTAKA KIMURA; JUN-ICHIRO KOMURA; YOSHINORI MIYAMURA; AKIHIRO KURISHITA; MASAO S. SASAKI; HIROMU SAKURAI; TETSUYA ONO

doi:10.1269/jrr.39.271

RIICHI TAWA, YUTAKA KIMURA, JUN-ICHIRO KOMURA, YOSHINORI MIYAMURA, AKIHIRO KURISHITA, MASAO S. SASAKI, HIROMU SAKURAI, TETSUYA ONO

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Keywords: X-rays, Tissues, HPLC, 5-methyldeoxycytidine, Demethylation

JOURNAL FREE ACCESS

1998 Volume 39 Issue 4 Pages 271-278

DOI https://doi.org/10.1269/jrr.39.271

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Abstract

Effects of ionizing radiation on the level of genomic DNA methylation in liver, brain and spleen of mouse as well as in two kinds of cultured cells were examined by high-performance liquid chromatography. Ten Gy of whole body X-radiation reduced the 5-methyldeoxycytidine contents by about 40% within 8 hours after irradiation in liver. Similar effects were observed at 4 or 7 Gy of X-ray irradiation. However, no such change was detected in brain, spleen and cultured cells. The data indicate that radiation-induced alteration in genomic DNA methylation is not ubiquitous among different tissues and cells.

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