Abstract
We have developed a new technique for geneexpression profiling called HiCEP. This method can analyze approximately80% of the genes that are expressed in a cell sample. A peak profilegenerated by HiCEP analysis represents the complete pattern of geneexpression in a sample under given conditions. Comparisons betweenprofiles made under different conditions (time after treatment, knock-outanimals etc) have revealed many new genes in our laboratory.HiCEP is different from other expression profiling methods in that it doesnot require any sequence information prior to the analysis, it is sensitiveand its results are highly reproducible. We are now building a database ofHiCEP peaks that will allow us to quickly identify the gene correspondingto a given peak. One such database based on expression profiling data of amouse embryonic stem cell line will be made available to the public soon.This kind of database should be useful in biological research on geneticdeficiencies, changes in response to the environment etc. [J Radiat Res 44:391 (2003)]
