Abstract
C3H/He mice develop acute myeloid leukemia (AML) after whole-body irradiation, and typical chromosome 2 deletions are found in the leukemic cells. We have examined the frequencies of the AML-type deletions in primitive hematopoietic cells that could be the targets of the leukemogenesis. Ten-week male C3H/He mice were exposed to 3Gy x-rays and sacrificed after certain periods of time. Bone marrow cells were collected from femora and a single-cell suspension from each animal was divided into two parts. One part was processed with MACS to obtain Lin+ and Lin−Sca1+ cells. Those cells were analyzed with FISH for the AML-type deletions. The other part of the cell suspension was cultured in methyl cellulose media, and metaphase spreads were prepared from each growing colony. Results obtained so far for 1 day post irradiation show that approximately 3% of the cells carried the AML-type deletions in both Lin+ and Lin−Sca1+ subpopulations while metaphases in GM colonies were mostly of normal karyotypes except for a small number of non-clonal aberrations. [J Radiat Res 44:396 (2003)]
