Effects of the ectopic expression and the reduction of expression of dRad30A, dRad30B and dRev1 genes in Drosophila

Abstract

RNAi is a powerful tool for the analysis of gene function in vivo. The "Y-family" of DNA polymerases are characterized in terms of their low-fidelity synthesis on undamaged DNA and their ability to bypass DNA lesions in vitro which normally block replication by previously known polymerases.To analyze the function of these genes in vivo, we have tried to establish a transgenic line which can overexpress RNAi construct of Drosophila "Y-family" DNA polymerasegenes, Rad30A, Rad30B and Rev1. This trial, howevfer, did not showed satisfactory result, because of the low efficiency in the repression of the level of protein. Thus, we have established new RNAi transgenic lines using improved RNAi vector and in this paper we will report the results of their characterization. [J Radiat Res 44:404 (2003)]