Effects of Point Mutations in Ku80 Proteins on Ku-dependent DNA Repair

Abstract

Ku, a heterodimer of Ku70 and Ku80, plays a key role in multiple nuclear processes, e.g. DNA repair, chromosome maintenance, and transcription regulation. We generated cell lines expressing the human Ku80 tagged with the green fluorescent protein color variants. The tagged Ku80 complements a deficiency in Ku-deficient mammalian cells. Therefore, these cells should prove useful in the analysis of the function of Ku in living cells. We also established stable cell lines expressing Ku80-mutants to which mutations were introduced by the site-directed mutagenesis technique. [J Radiat Res 44:412-413 (2003)]