Abstract
Mouse lymph node cell (M10) is highly sensitive against X-ray irradiation. Growth of this cell depended on the UV-rays irradiation. The cell growth was repressed in proportion to the irradiation time. Dynamic changes of 597 genes after the UV-B (310 nm) irradiation were investigated on DNA array membranes (Atlas #7741-1,CLONTECH) using P-33 labeling probe. Radioactivities obtained from imaging analyzer (BAS1500, Fuji film co. ltd.) were analyzed using software of ArrayGauge. The expression of mRNA was further analyzed by EX-ARRAY program. Seven housekeeping genes were used as standards. Irradiations for 10-second, 20-second and 30-second were done, and changes in the gene expression after the cultivation for 2 h were compared with cells without irradiation. As for a group of activated genes, UV-rays reached more than 80 kinds in 597 genes for 10-second irradiation, while 110 kinds as for a group of repression genes. Activated genes decreased due to the irradiation time, and it became clear that repression genes increased in proportion to the irradiation time. [J Radiat Res 44:415 (2003)]
