Abstract
We previously showed that cyclobutane pyrimidine dimer (CPD) photolyase was deficient in UVB-sensitive rice, and that the CPD photolyase deficiency might result from a mutation of CPD photolyase. In this study, we carried out the genetic analysis of correlation between UVB-sensitivity and mutation of CPD photolyase using F2 plants generated by reciprocally crossing UVB-resistant Sasanishiki (Japonica rice cultivar) and UVB-sensitive Surjamkhi (Indica rice cultivar), and F3 lines generated by self-fertilizing of F2 plants. The CPD photolyase activity in Surjamkhi was significantly lower than that in Sasanishiki. There were two variations in the deduced amino acid sequence of CPD photolyase in these two cultivars. We investigated the UVB sensitivity in F2 plants and the genotype of CPD photolyase in F3 lines. Consequently, we found that strong correlation exists between the UVB-sensitivity and mutation of CPD photolyase in UV-sensitive and -resistant rice cultivars. [J Radiat Res 44:416 (2003)]
