Abstract
We investigated the p53 signal pathway by hypergravity in human glioblastoma cell line, A172. Hypergravity (20g) induced the accumulation and the phosphorylation of p53 at 3h. But hypergravity did not significantly induced Waf-1 or Bax. Then, we examined the expression of genes by using Human Oligo Chip 30K (Hitachi Software Engineering Co., Ltd.). This DNA chip contained oligo DNA from 30,000 genes. Any of p53 inducible genes did not induced, but some genes concerned with cell signaling pathway or cytoskeleton of the cell induced by hypergravity (20g, 6h). We considered that the p53 signal pathway by hypergravity is different from other p53 signal pathway like DNA damage signal. DNA chip revealed that cells induced many gene expressions to adapt the environment of hypergravity. [J Radiat Res 44:418 (2003)]
