Abstract
Reactive oxygen species (ROS) give rise to various types of oxidative damage and some responses such as activation of stress responsive transcription and/or repair factors in cells. Proteome analysis using 2D-PAGE and peptide mass fingerprinting is suitable for the study on the post-translational modifications of proteins. When glial cells were exposed to hydroperoxide, the relative abundance of 9 spots changed on 2D gels, as compared with control gels. MALDI-TOF MS analysis after in-gel digestion revealed that these spots corresponded to at least 3 pairs of proteins. These pairs of protein spots had different isoelectric points each other and were identified as peroxiredoxin II, peroxiredoxin III and calpactin. It was suggested that peroxiredoxin II, peroxiredoxin III and calpactin were changed to their modified forms of a different isoelectric point by hydroperoxide. In the present work, we studied also the variant expressions of proteins caused by irradiation, and will discuss the effects of oxidative stress on proteins in the presentation. [J Radiat Res 44:421 (2003)]
