Abstract
Ionizing radiation generates various types of DNA lesions such as base damage and strand breaks. The spectra of DNA damage induced by high LET radiation has been relatively less clarified than that by low LET radiation such as gamma or X rays. In the present study, we have quantitated isolated and clustered oxidized pyrimidine and purine lesions and double strand breaks generated high LET particles. Lambda phage DNA or pDEL plasmid DNA was irradiated with C or Fe ions in phosphate or Tris buffer using the HIMAC accelerator at NIRS in Chiba. After irradiation, the DNA treated without or with endonuclease III or hOGG1 was subjected to pulse field gel electrophoresis to quantitate radiation-induced double strand breaks and clustered oxidized base lesions. The DNA was also assayed for oxidized base lesions using the aldehyde reactive probe (ARP) method. [J Radiat Res 44:436 (2003)]
