Abstract
Werner syndrome (WS) is autosomal recessive disorder exhibits features of premature aging.The precise role of werner protein (WRN) remain to be determined. In order to reveal the causes of abnormalities, We introduced human normal chromosome 8 codes WRN gene into the ws cell immortalized by htert.Ws cell has previously been shown to have hypersensitivity to 4-nitroquinoline1-oxide (4NQO), and cis-platinum (II) diamine dichloride (CDDP), hydroxyurea (HU) and have increased chromosomal instability after X-ray irradiated. So we tested these agents and treatment. the results show the hyper sensitivity to 4nqo and cddp, chromosomal instability were complemented. And hu was partially complemented. These findings suggest WRN protein has functions concerning recombinational DNA repair. Further more, these complementation have not seen in the ws cell has human normal chromosome 8 immortalized by SV40. This may be the result of occurring further mutation caused by deficient of WRN protein. [J Radiat Res 44:439 (2003)]
