Cell Inactivation and Double Strand Breaks Induction in V79 cells by Monochromatic X rays on Phosphorus K-shell Absorption peak

Abstract

Monochromatic X-rays at the energy (2.153 keV) of K-shell absorption peak of phosphorus are strongly absorbed by phosphorus K-shell and followed by Auger processes. We irradiated V79 (Chinese hamster) cells by X-rays of 2.153 keV (peak), and 2.147 keV (low), which is 6 eV lower off the peak. Survival curves revealed that the doses at the survival 10% were 0.83 times less at the peak than at the low; namely, peak X-rays inactivated the cells 1.20 times efficiently. Induction and repair of DSBs were measured with Pulse Field Gel Electrophoresis (PFGE). The percentage of DNA fragments size of below 4.6 Mbp was adopted as measure of DSB amount: the 2.153 keV induced DSBs 1.40 times higher, although the rejoining of induced DSBs were 0.85 times less compare to the 2.147 keV. [J Radiat Res 44:440 (2003)]