Abstract
For the performance test of the synchrotron X-ray microbeam system, especially accuracy and repeatability of the positioning in the system, the cell irradiation experiments were performed and microbeam-irradiated cells was observed after immunostaining with gamma-H2AX antibody. The dishes specially designed for microbeam experiments are composed of a thin Mylar bottom and a set of solid frames of stainless steel. Human fibroblast cells were inoculated to the dish and incubated to attach the surface of Mylar bottom. Before irradiation cell nuclei were stained with Hoechst33258 and identified with the fluorescence microscope equipped in the system. Selected cells were individually irradiated with X-ray microbeam and the positions of the irradiated cells were automatically memorized. After immunostaining treatment, the dish was set again to the system to revisit at the poison of the irradiated cells. All the irradiated-cells could be observed at the revisited position, and could be distinguished from surrounding unirradiated cells by their high yield of fluorescence. [J Radiat Res 44:444 (2003)]
