Abstract
Purpose. The report is the study of the effect of nanosecond impulse irradiation of X-ray by SINUS-150 accelerator on tumor and normal cells proliferation. Materials and Methods. The SINUS-150 accelerator (peak electron energy 280 keV, peak beam current 4 kA, irradiation pulse rates is 6-25 Hz, pulse duration 4 ns) was developed by our Institute. The X-ray impulse irradiation dose rate is up to 0.05 mGy/pulse, the energy range of photon beam is 80-160 keV, the specific depth of penetration is about 7 cm. Total irradiation dose ranged between 36 and 144mGy. Cell proliferation was assayed by 3H-thymidine incorporation to DNA. Results. The 3H-thymidine incorporation of different tumor cells lines was almost completely inhibited by exposing to pulse irradiation with some different impulse frequencies. P-815 mastocytoma cells treatment with pulse irradiation resulted in a significant decrease of cell proliferation by 97%. On the contrary, no any inhibitory effect was detected after their exposition with the same doses of stationary irradiation. Inhibitory effect of pulse low dose X-ray on cell proliferation was depended on impulse frequency, but not dose of irradiation. Impulse X-ray had no effect on lymphocyte proliferation. Conclusion. The nanosecond impulse irradiation of low dose X-ray brought about high inhibition effect on tumor cells proliferation without damage of normal cells.
