Abstract
We reported previously that adaptive response to low dose irradiation decreased with aging. In order to investigate its mechanisms, we searched the variant expressions of proteins caused by high or low dose irradiation in cells from variously aged rats. Proteomics using 2D-PAGE and peptide mass fingerprinting is suitable for the study on both the expressions and the post-translational modifications of proteins. Alterations in the relative intensity of protein spots appearing on proteome profiles were analyzed using PDQuest software (Bio-Rad) and identifications of protein spots were performed using MALDI-TOF MS analysis. When glial cells cultured from young rats were irradiated at the dose of 5 Gy, the relative abundance of 2 spots increased on 2D gels, as compared with control gels. These spots corresponded to peroxiredoxin II and latexin. While in cells from aged rats, both peroxiredoxin II and latexin were not increased significantly by 5 Gy-irradiation. In the present work, we also studied the protein spots phosphorylated by irradiation, and will discuss the effects of aging on cell responses to radiation in the presentation.
