Abstract
(Purpose) Hyperthermia was performed at over 42.5C in clinicaly, but we find that below 42.5C as 41C still has effects of enhanced the anti-tumor effect of the drug and increase of the immunological activity. (Materials and Methods) SCC-VII tumor cells were transplanted subcutaneously in the thigh of C3H male mice 6 weeks old. For heat treatment of the tumor, a thermostatic circulating water bath(41C and 42C,30min) was used on ten mice in each group which were control group, drug alone group, heat alone group and drug plus heat group. Also we measured number of the white blood cells and lymphocyte cells, and activate of immunological activity as NK cells activity using Cr-51 labeled EL-4 cells assay. Also we measured the drug uptake in the tumor using HPLC units. (Results and Discussion) Mild-hyperthermia has effect of enhance the anti-tumor effect by increase of drug uptake due to the increase of blood flow in the tumor when combined with drug and mild-hyperthermia. The number of white blood cells and lymphocyte cells and NK cells activity as immunological activity were increased after hyperthermia. These results suggest that mild-hyperthermia can reduce the side effects due to the reduction of the drug doses and increase of immunological activity can maintain the QOL levels for patients.
