Construction of various systems to analyse effects of DNA double strand break (DSB) in mammalian cells.

Abstract

It is well known that irradiation cause many effects to organismes, like mutation, cell death or tumor. The DSB is major DNA damage by irradiation and, thus this type of damage should be involved in these effects. In order to elucidate the detail effect of DSBs in mammalian cells, we are constructing varions systems to: 1) visualize the DSB repair by introducing fluorescence marker near to the DSB site, 2) isolate proteins which associate to the DSBs site through or after the repair, 3) follow expression pattern of a gene near to the DSB site after the repair. The current data would be presented.