Host: The Japan Radiation Research Society
Co-host: Asian Association for Radiation Research
Ionizing radiation and reactive oxygen species produce various types of oxidative damage to DNA, which cause mutations in cells. Bacteria and eukaryotes have DNA repair systems to prevent mutations. Oxidative base damages are principally repaired by base excision repair (BER) mechanisms. However, there are no indications that oxidative damages are directly reversed on the DNA strand. In this study, we examined whether oxidative damages are directly reversed on the DNA strand. We used pUB3 plasmid irradiated with gamma-rays and subsequently treated with a reducig agent, dithiolthreitol (DTT). E. coli cells were transformed with the pUB3. As a result, the transformation efficiency of pUB3 treated with DTT was higher than that of pUB3 without DTT treatment. We are currently investigating the mutation spectra of the pUB3.
