Abstract
Mouse lymph node cell (M10) is highly sensitive to X-ray irradiation. This cell is also sensitive against UV-ray irradiation. Growth of this cell depended on the UV-rays (310 nm, band B) irradiation, and the cell growth was suppressed in proportion to the UV irradiation time. UV irradiation doses (10, 20, 30 and 60sec) showed the induction of cell apoptosis and necrosis. Cell sorting analysis distinguished the difference of the apoptosis and the necrosis of M10 cells. Apoptotic cells increased in proportion to UV-irradiation. We also analyzed the gene expression in the UV-irradiated cells using DNA array technology. Dynamic changes of 597 genes after the UV-B (310 nm) irradiation of three doses (10, 20 and 30sec) were investigated on DNA array membranes. Radioactivities obtained from imaging analyzer were analyzed using software of ArrayGauge. The expression of mRNA was further analyzed by EX-ARRAY program. Time course of the gene expression was investigated at 2h and 5h after the irradiation. The expression of almost genes reduced in proportion to the doses. The comparison of the gene expression with the parental cells (L5178Y) demonstrated the remarkable activation of homeobox gene (Hox-2.4) and serine (or cysteine) proteinase inhibitor, clade B member 6 (Serpinb6).
