Abstract
Micronucleus formation is used as a simple method to assess the biological effects of radiation instead of chromosomal aberration. Micornucleus in binuclear cells during the culture with cytochalasin B derived from lymphocytes in peripheral blood cells are usually observed. It is applied to a method of bioassay to assess radiation dose. We used it as one of the indices for the assessment of the effects of low dose/low dose rate radiation because the assessment needs a number of data for statistical use. The cell culture under irradiation continues growth since the cells are cultured for a long time without serious damage. There is possibly a difference in the micronucleus formation rate between the binuclear cells from growing cultured cells and those from lymphocytes derived from one step culture. There is because lymphocytes in peripheral blood originally may have no cells with micronuclei whereas some cells in the growing culture have them. It is alos possible that cells with micronucleus will divide further. The difference was assessed with a model based on recurrence relation under several simple hypotheses.
