Host: The Japan Radiation Research Society
Co-host: Asian Association for Radiation Research
The effect of temperature and duration of storage the blood samples on PCC index following high dose exposure Gotoh, Eisuke (egotoh@nih.go.jp)After high dose exposure accident, the absorbed dose should be determined as quick as possible for save the life of victims. The easy and simple protocol of biodosimetry using Giemsa stained prematurely condensed chromosomes has been reported on the previous JRRS meeting. In the present report, we investigated the how the storing temperature/duration of blood samples for chromosome analysis will be affected on PCC index following high dose exposure accident. In the case of the blood was kept at 4oC, the PCC index was drastically decreased, particularly the case of the blood was exposed over 30 Gy. Therefore, the blood sample should be avoided to be store at cool temperature during shipping the blood until start culture of lymphocyte for chromosome analysis.