Host: The Japan Radiation Research Society
Co-host: Asian Association for Radiation Research
Biological effects of various magnetic fields are not clear in spite of a large number of studies. In a practical sense, there are a number of frequencies and strength of magnetic field in our living environment. Therefore, investigations of biological effects of such complex magnetic fields are required. In this study, effects on cell growth and neurite outgrowth of complex magnetic field with static and 50Hz were investigated using PC12 cell. Cell growth was measured using WST-1 (Roche Diagnostics Co.). 150 µl of cell suspension (5×104 cells/ml) poured in two 96-well plates. One plate was incubated under complex magnetic field with 5T static and 1mT 50Hz components and the other was incubated in conventional incubator. 15 µl of WST-1 solution added to 8 wells and absorption of 415 nm was measured after 3hr incubation following days. Result shows no significant difference on cell growth between complex magnetic field exposure and non-exposure cells. Neurite outgrowth was examined using NS-200 (CHEMICON Int. Co.). Cell suspension was poured into membrane insert for two 12-well plates with RPMI 1640 with 10% FCS, 5% horse serum and NGF (100ng/ml) and plates were treated as cell growth experiment and after 24 hr incubation, cells were fixed with methanol and stained with dye. Neurite outgrowth was estimated by microscopic observation. No significant difference was observed between exposed and control cells. These results show that lack of the biological effects of a complex magnetic field with 5T static and 1mT 50Hz magnetic field.
