Host: The Japan Radiation Research Society
Co-host: Asian Association for Radiation Research
Somatic rearrangements of the tyrosine kinase receptor RET have known to involve radiation-associated papillary thyroid carcinoma. Major types of rearrangements are RET/PTC 1, 2 and 3 that is more than 90% of PTC with RET rearrangements. We investigated the induction of RET rearrangements in human normal thyroid cell by gamma radiation. Human normal thyroid cells were exposed 0, 1, 3, 5, and 10Gy gamma radiation and harvested 2 to 9 days after irradiation. The induction of RET/PTC1, 2 and 3 were analyzed by reverse transcription-polymerase chain reaction and sequencing. No RET/PTC induction observed in cells harvested 2days. RET/PTC1 and RET/PTC3 detected 5 to 9 days after irradiation. RET/PTC1 was more prevalent than RET/PTC3 after each dose and RET/PTC1 was generated dose-dependently by gamma radiation. But, RET/PTC2 was not generated. This study demonstrated a dose-dependent induction of RET/PTC1 in human normal thyroid cells by gamma radiation. Theses results suggested an immediate association between RET rearrangements and gamma radiation exposure in human normal thyroid cells.
