Abstract
We investigated the p53 signal pathway of radiation by phosphorylation at serine 20 in human lung adenocarcinoma cell line, H1299. We made two mutations of p53 at serine 20, S20A which does not phosphorylate and S20D which is a mimic of phosphorylation. The cells with wild type p53 or S20A induced growth inhibition. On the other hand, the cells with S20D did not induce the inhibition of the cell growth. Then, we examined the expression of genes in the cells with S20D by using the Human Oligo Chip 30K (DNA Chip Research Inc.) contained oligo DNA from 30,000 genes. S20D induced some genes, but did not induce the typical radiation inducing genes. We considered that the phosphorylation of p53 at serine 20 alone did not affect the p53 signal genes by radiation, and other modifications of p53 might concern with the signal transduction.
