Abstract
BALB/c mice show susceptibility to radiation-induction of lymphomas, while STS mice show resistance. Resistance to lymphomagenesis has been associated with the middle-proximal domain of STS-derived chromosome 4. Using DNA microarrays, gene expression profiling has been performed in different C.S congenic animals containing the STS alleles in this domain in comparison with the background strain BALB/c. The analysis revealed 7 and 12 spots with more than 2 of the median of ratios (Cy5/Cy3) in C.S7-86 and C.S302-9 animals, respectively. Among these spots 5 were common, and only 2 were on chromosome 4. Other spots are speculated to be downstream of the STS alleles in the congenics. To exclude false-positive signals possibly caused by different dye-labeling efficiency, comparison of the expression levels of these genes from the congenics to those from the BALB/c is presently under way using a real-time PCR analysis.
