Abstract
We performed basal examination of ABM-FD (Freezing dryness ploysaccaharides, Agaricus Blazei Muril l; Himematsutake) about the safety and the protection effect of whole body X-ray irradiation in normal mice. In addition, combination effects with X-ray irradiation were investigated in Meth A tumor-bearing mice. The oral administration with safety for ABM-FD massive dose (7,200mg/kg/day x 4), there was not a change to mention specially in general findings after administration (body weight and organ findings in autopsy). However, increased 2.67 times (p<0.001) for control (distilled water administration) in ABM-FD administrated group was present in WBC count. Furthermore, the increase was found in an incorporation of 3H-thymidine of Con-A and LPS addition lymphocyte by the mitogen reaction of splenic lymphocytes in ABM-FD administrated group. The ABM-FD (680mg/kg/day x 15-18) administrated groups in which performed immediately after irradiation administrated group showed survival rate of 66%, but 6 days after irradiation administrated group and control group for survival rate 33%, it is a clear effect was found in protection from whole body exposure (8.0 Gy). The oral administration of ABM-FD (960mg/kg/day x 7-10) and X-ray irradiation (3 Gy/day x 4) moderately inhibited the growth of Meth A tumor cells implanted s.c. in mice. Development of implanted tumor was strongly inhibited by the combination of ABM-FD and irradiation. These result suggest that the radiation exposure protection effect of AMB-FD inhibits bone marrow death not action as radical scavenger.
