Abstract
(Background) FGFs are currently composed of 22 members in human. They have important roles in numerous biological events such as angiogenesis, wound repair and so on. Recently several FGFs have been put to the clinical trials. FGF1 has more potential because it can react on all FGFRs. However, there are a few reports about the efficacy of FGF1. (Aim) An efficacy of FGF1 on radiation-induced intestinal injuries was investigated to realize an appropriate condition of its administration. (Methods) For crypt regeneration assay (CRA), Balb/c mice was exposed to 8 Gy irradiation under several conditions of i.p. administration of FGF1. Crypt cell apoptosis was investigated for the mice 24 hours after irradiation by Tunel assay. (Result) Increase of crypt survival was shown with FGF1 administration even after irradiation by CRA. Especially, maximum experimental dose, 100μg, had most beneficial effect. FGF1 decreased cumulative apoptosis of irradiated crypts in a dose-dependent manner. The LD50/6 (11.5Gy) was not increased by the pretreatment of FGF1, however the jejunum tissue damage was slighter that control. (Discussion) These findings showed that FGF1 had an effect on the radiation-induced intestinal damage in a dose-dependent manner. The jejunum tissue damage was histopathologically improved by FGF1, although LD50/6 was not increased. The death of highly irradiated mice might be caused by not only intestine damage, but also multiple organ damage. Therefore it was supposed that FGF1 had the potential to improve the intestine damage.
