Abstract
Genetical technique in Drosophila melanogaster can hold unevenness of spontaneous mutation frequency (about 0.3%) in check small, because of the leveling of genetical background. This technical advantage made clear mutation frequency reduction at the low dose, low dose rate irradiation than spontaneous mutation frequency. Irradiation to the Drosophila immature sperm using gamma ray of 0.5 mGy in one minute from the 60Co reduced the frequency of the sex-linked recessive mutation up to 0.1% (p<0.05). To explain this effect, the transcript profiles were evaluated using oligonucleotide arrays from the same stage embryos which have an immature sperm. We report here, a set of 160 genes, including hsp 70A gene, which is known as stress response protein gene, with transcripts that were affected by this level. However, genetic expression was not able to observe the significant change of expression of the gene group, (e.g. mei-41, p53) which participated in repair, cell cycle or apoptosis and were affected by high dose, high dose rate irradiation. We suggested that the expression of the gene group as mei-41 and p53, have minimum dose to response.
